CD355 (CRTAM) Monoclonal Antibody (Cr24.1), PE-Cyanine7, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen 25-3559-41

Product Code. 16340774

Description

This monoclonal antibody Cr24.1 reacts with human CD355, also known as Class-I MHC-restricted T-cell-associated molecule (CRTAM). CD355 usually exists as a homodimer, however, it forms a heterodimer with its ligand Necl-2. CD355 is transiently expressed by activated NK, NKT, gamma delta T, and CD8+ T cells. CD355 is also expressed by Purkinje neurons. Human CD355 is involved in immunosurveillance of cancer cells, gamma delta T cell survival, cell polarity, retention of lymphocytes in lymph nodes, promotion of cytotoxicity in NK cells, and IFN gamma secretion from CD8+ T cells. Cr24.1 has been reported to have antagonist activity. This Cr24.1 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Filtration: 0.2 μm post-manufacturing filtered. The CRTAM gene is upregulated in CD4-positive and CD8-positive T cells and encodes a type I transmembrane protein with V and C1-like Ig domains.

Specifications

• Antigen: CD355 (CRTAM)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide
• Gene Accession No.: O95727
• Gene Symbols: CRTAM
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 56253
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: Cr24.1
• Conjugate: PE-Cyanine7
• Gene: CRTAM
• Gene Alias: CD355; class I MHC restricted T cell associated molecule; class-I MHC-restricted T cell associated molecule; class-I MHC-restricted T-cell-associated molecule; cytotoxic and regulatory T cell molecule
• Host Species: Mouse
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.