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CD45R (B220) Monoclonal Antibody (RA3-6B2), eFluor™ 615, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Invitrogen 42-0452-80
Description
Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen. Applications Reported: This RA3-6B2 antibody has been reported for use in immunohistochemical staining of frozen tissue sections (IHC-F), and immunocytochemistry. Applications Tested: This RA3-6B2 antibody has been tested by immunohistochemistry on frozen mouse spleen at less than or equal to 1 μg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor™ 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 μm post-manufacturing filtered.
CD45R is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling.Specifications
CD45R (B220) | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2 | |
P08575, P06800 | |
PTPRC | |
Affinity chromatography | |
RUO | |
19264, 5788 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen) | |
RA3-6B2 | |
eFluor 615 | |
PTPRC | |
B220, CD45R, Cd45, Ly-5, Lyt-4, T200, Cluster designation 45R | |
Rat | |
25 μg | |
Primary | |
Human, Mouse | |
Antibody | |
IgG2a κ |
For Research Use Only.