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CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), Biotin, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Invitrogen 13-0495-80
Description
Description: The eBioGoH3 monoclonal antibody reacts with mouse and human CD49f, also known as integrin alpha 6, very late activation antigen 6 (VLA-6 alpha chain), and platelet gpIc. CD49f is a 120 kD transmembrane protein. CD49f associates with CD29, the integrin beta 1 chain, to form the VLA-6 complex; CD49f also associates with CD104, the integrin beta 4 chain, to form the alpha 6 beta 4 complex. CD49f is expressed primarily on T cells, monocytes, platelets, epithelial and endothelial cells. CD49f expression has also been found on germinal center B cells. The eBioGoH3 antibody is cross-reactive to integrin alpha 6 on human, mouse and bovine cells. This antibody has also been reported to have functional activity in blocking the binding of integrin alpha 6 to laminin. Applications Reported: This eBioGoH3 (GoH3) antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections. Applications Tested: This eBioGoH3 (GoH3) antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.
2 μm post-manufacturing filtered. The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene.Specifications
CD49f (Integrin alpha 6) | |
Monoclonal | |
0.5 mg/mL | |
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2 | |
P23229, Q61739 | |
ITGA6 | |
Affinity chromatography | |
RUO | |
16403, 3655 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry, Immunohistochemistry (Frozen) | |
eBioGoH3 (GoH3) | |
Biotin | |
ITGA6 | |
CD49f, DKFZp686J01244, FLJ18737, ITGA6B, VLA-6 | |
Rat | |
25 μg | |
Primary | |
Human, Mouse | |
Antibody | |
IgG2a κ |
For Research Use Only.