CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), Functional Grade, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Invitrogen 16-0903-85

Product Code. 15268527

Description

Description: The 30-H12 monoclonal antibody reacts with mouse CD90.2, also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/J, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 30-H12. CD90 is involved in regulation of adhesion and signal transduction by T cells. Applications Reported: The 30-H12 antibody has been reported for use in flow cytometric analysis. It has also been reported in in vivo and in vitro depletion. Applications Tested: The 30-H12 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

Specifications

• Antigen: CD90.2 (Thy-1.2)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P01831
• Gene Symbols: Thy1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 21838
• Content And Storage: 4° C
• Form: Liquid

• Applications: Flow Cytometry, Functional Assay
• Clone: 30-H12
• Conjugate: Functional Grade
• Gene: Thy1
• Gene Alias: CD90; FLJ33325; T25; Thy 1.2; Thy1; Thy-1; Thy-1 antigen; thy-1 membrane glycoprotein; Thy1.1; Thy1.2; Thy-1.2; thymus cell antigen 1, theta
• Host Species: Rat
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2b κ

For Research Use Only.