CD95 (APO-1/Fas) Monoclonal Antibody (EOS9.1), Functional Grade, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
17121102	Invitrogen 16-0958-38	5 mg
17101102	Invitrogen 16-0958-025	25 mg
17111102	Invitrogen 16-0958-050	50 mg

Description

The EOS9.1 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. EOS9.1 does not block binding of DX2, another antibody specific for human CD95. Applications Reported: The EOS9.1 antibody has been reported for use in flow cytometric analysis. EOS9.1 is also effective in inducing apoptosis in in vitro functional studies. Applications Tested: The EOS9.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/ μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD95 (APO-1/Fas) Monoclonal antibody specifically detects CD95 (APO-1/Fas) in Human samples. It is validated for Flow Cytometry, Functional Assay

Specifications

• Antigen: CD95 (APO-1/Fas)
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with no preservative; pH 7.2
• Gene Alias: AI196731; ALPS1A; APO1; APO-1; Apo1 antigen; apo-1 antigen; APO-1 cell surface antigen; Apo-1 Fas; apoptosis antigen 1; apoptosis signaling receptor FAS; apoptosis-mediating surface antigen FAS; APT1; CD95; CD95 antigen; fas; Fas (TNF receptor superfamily member 6); Fas (TNF receptor superfamily member); Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; Fas antigen (ATP1); Fas cell surface death receptor; Fas receptor; FAS1; FASLG receptor; FASTM; lpr; lymphoproliferation; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; TNF receptor superfamily member 6; TNFR6; Tnfrsf6; Tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6
• Isotype: IgM κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: EOS9.1
• Applications: Flow Cytometry, Functional Assay
• Conjugate: Functional Grade
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: P25445
• Gene ID (Entrez): 355
• Primary or Secondary: Primary
• Content And Storage: 4° C
• Gene: FAS