Invitrogen™ CDK2/cyclin A1 Recombinant Human Protein

CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. Recombinant full-length human CDK2 and CyclinA1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.

Marque:  Invitrogen™ PV6289

Code produit. 15408943

Description

CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition.

• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Spécification

• For Use With (Application): Enzyme and Protein Activity Assay, Industrial and Applied Science, Kinase Assay, Kinase Biology, Kinase Proteins and Expression Reagents, Pharma and Biopharma, Protein Assays and Analysis, Protein Biology, Target and Lead Identification and Validation
• Molecular Weight (g/mol): 60.2/78.5kDa
• Storage Requirements: Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.
• Species: Human
• Protein Tag: GST Tag

• Formulation: 50mM Tris-HCl pH 7.5, 150mM NaCl, 0.25mM DTT, 10mM glutathione, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol
• Quantity: 10μg
• Gene Alias: CDK2: p33(CDK2) CyclinA1: CCNA1
• Recombinant: Recombinant