Invitrogen™ Human CDK4/cyclin D3, GST Tag Recombinant Protein

Human CDK4/cyclin D3, GST Tag Recombinant Protein

Brand:  Invitrogen™ A33736

Product Code. 17188845

Description

CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivities in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.

• Validated for use with the Adapta™ assay
• Available in multiple pack sizes for convenient use

Specifications

• Accession Number: CDK4: NP_000066.1, Cyclin D3: NP_001751.1
• Molecular Weight (g/mol): 60.0/58.8 kDa
• KinaseFamily: CDKs and CHKs
• Species: Human
• Protein Tag: GST Tag
• Shipping Condition: Dry Ice
• Protein Form: Full Length, Recombinant, Active

• For Use With (Application): Kinase Assay
• Quantity: 1 mg
• Protein Length: CDK4: Full Length, Cyclin D3: Full Length
• Recombinant: Recombinant
• Content And Storage: Storage Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
• Expression System: Insect
• Form: Liquid