Invitrogen™ Human CDK4/cyclin D3, GST Tag Recombinant Protein

Recombinant Protein

Brand:  Invitrogen™ A33736

Product Code. 17188845

Description

CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.

Specifications

• Accession Number: P11802
• For Use With (Application): Kinase Assay
• Gene ID (Entrez): 1019
• Name: Human CDK4/cyclin D3, GST Tag
• Gene Alias: Cdk4; Cell division protein kinase 4; CMM3; Crk3; cyclin dependent kinase 4; cyclin-dependent kinase 4; MGC14458; PSK-J3; serine/threonine kinase; Unknown (protein for MGC:133903)
• KinaseFamily: CDKs and CHKs
• Species: Human
• Protein Tag: GST-tag
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Expression System: Insect cells
• Form: Liquid

• Concentration: See Label
• Formulation: 50 mM tris HCl with 0.1 mM EDTA, 0.1 mM PMSF, 0.25 mM DTT, 10 mM glutathione, 150 mM NaCl, 25% glycerol and no preservative; pH 7.5
• Molecular Weight (g/mol): 60.0-58.8 kDa
• Quantity: 1 mg
• Common Name: CDK4
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: CDK4: 1-303, Cyclin D3: 1-292
• Shipping Condition: Dry Ice
• Protein Form: Full Length, Recombinant, Active