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Invitrogen™ CellEvent™ Senescence Green Detection Kit

CellEvent™ Senescence Green Detection Kit consists of a fluorescent probe and optimized buffer that enable the image-based detection of senescent cells

£286.00 - £518.00

Specifications

Cell Type Mammalian Cells
Detection Method Fluorescence
For Use With (Equipment) Fluorescence Microscope, High Content Analysis Instrument
Product Type Senescence Green Detection Kit
Format 96-well plate
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
16232102
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Invitrogen™
C10850
25 μL
£286.00
Each
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16376622
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Invitrogen™
C10851
100 μL
£518.00
100µL
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Description

Description

This fluorescein-based probe contains two galactoside moieties, making it a target for β-galactosidase. Activation of β-galactosidase is commonly used as a biomarker for senescent cells. This hydrolase enzyme resides in lysosomes and converts β-galactosides into monosaccharides under acidic pH conditions. The enzymatically cleaved product is retained within the cell due to covalent binding of intracellular proteins and emits a fluorogenic signal that has absorption/emission maxima of 490/514 nm.

Kit features include:
• Reliable and quick fluorescent detection of senescent cells
• Greater sensitivity than traditional colorimetric x-gal
• Fluorescent signal is well retained and detected using standard Alexa Fluor™ 488/FITC filter sets
• Multiplex-enabled—fluorescent senescence probe can be multiplexed

Due to a limited replicative lifespan, normal cells enter cell cycle arrest, also known as cellular senescence. While in this senescent phase the cells remain metabolically active without undergoing cell death or division. They adopt a specific phenotypic that includes the appearance of multiple nuclei, increased vacuolization, expression of pH-dependent β-galactosidase, and morphological enlargement and extension. Senescence, through a variety of mechanisms, may play a role in tumor suppression, tumor progression, aging, and tissue repair.


Activation of β-galactosidase is commonly used as a biomarker for senescent cells. This hydrolase enzyme resides in lysosomes and converts β-galactosides into monosaccharides under acidic pH conditions. The activity is optimal at lysosomal pH 4, but conventional assays measure at pH 6. It has been shown that normalized β-galactosidase activity is twice as high in senescent cells as in pre-senescent cells regardless of the pH value used for testing.


A colorimetric substrate for β-galactosidase, 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (x-gal), has long been used to detect metabolic activity in cells in vitro. However, its use is limited due to inconsistent signal, length of assay, and inability to multiplex.


Therefore, we have developed a sensitive, fluorescent substrate for β-galactosidase that can be used for the detection of senescent cells. The CellEvent™ Senescence Green probe is a fluorescein-based reagent that contains two galactoside moieties, making it a specific target of β-galactosidase. The enzyme- cleaved product is retained within the cell due to covalent binding of intracellular proteins and emits a fluorogenic signal that has absorption/emission maxima of 490/514 nm.

The CellEvent™ Senescent Green Kit provides CellEvent™ Senescent Green probe and an optimized buffer for the detection of senescent cells in fixed samples. This probe can be multiplexed with other fluorescent reagents compatible with paraformaldehyde fixation.

Specifications

Specifications

Mammalian Cells
Fluorescence Microscope, High Content Analysis Instrument
96-well plate
Approved for shipment at Room Temperature or on Wet Ice
Fluorescence
Senescence Green Detection Kit
CellEvent™
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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