Molecular Probes™ CellROX™ Deep Red Flow Cytometry Assay Kit

CellROX™ Deep Red Flow Cytometry Assay Kit

Brand:  Molecular Probes™ C10491

Product Code. 15246066

Description

The CellROX™ Deep Red Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX™ Deep Red Reagent, as well as SYTOX™ Blue dead cell stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS).

View a selection guide for all CellROX™ Reagents and Kits.

The CellROX™ Deep Red Flow Cytometry Assay Kit features:

Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS

Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines, allowing easy multiplexing with other reagents

Simple protocol—cells can be stained in complete media or other appropriate buffer, no need for serum free media

The CellROX™ Deep Red Detection Reagent is cell-permeable and non-fluorescent or very weakly fluorescent while in the reduced state. Upon oxidation, the reagent exhibits a strong fluorogenic signal that has absorption/emission maxima of 644/665 nm and remains localized in the cytoplasm. When used together with the included SYTOX™ Blue Dead Cell Stain, oxidatively stressed and non-stressed cells are reliably distinguished from dead cells by flow cytometry.

Specifications

• Cell Type: Mammalian Cells, Eukaryotic Cells
• Detection Method: Fluorescence
• For Use With (Equipment): Flow Cytometer
• Dye Type: CellROX™ Deep Red Reagent
• Format: Tube(s)
• Quantity: 100 Assays
• Solubility: DMSO (Dimethylsulfoxide)

• Content And Storage: Contains 1 vial CellROX™ Deep Red Reagent (25 μL), 1 vial SYTOX™ Blue Dead Cell Stain (100 μL), 2 vials N-acetylcysteine (10 mg per vial), 1 vial tert-butyl hydroperoxide (50 μL, 70% solution in water), and 1 vial DMSO (200 μL).
  
  Store kit at -5 to -30°C, protected from light. CellROX™ reagent is air sensitive.
• For Use With (Application): Flow Cytometry
• Product Type: Reagent
• Emission: CellROX™ Deep Red: 644⁄665, SYTOX™ blue: 444⁄480
• Product Line: CellROX™
• Shipping Condition: Wet Ice

Safety and Handling

Product Identifier

• CellROX Deep Red Flow Cytometry Assay Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 3
• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 2
• Serious eye damage/eye irritation Category 1
• Flammable liquid Category 3
• Germ cell mutagenicity Category 2
• Organic peroxide Type E & F
• Skin corrosion/irritation Category 1 B
• Skin sensitiser Category 1

Hazard Statement

• H226-Flammable liquid and vapour.
• H242-Heating may cause a fire.
• H302-Harmful if swallowed.
• H311-Toxic in contact with skin.
• H314-Causes severe skin burns and eye damage.
• H317-May cause an allergic skin reaction.
• H331-Toxic if inhaled.
• H341-Suspected of causing genetic defects.
• H411-Toxic to aquatic life with long lasting effects.

Precautionary Statement

• P201-Obtain special instructions before use.
• P273-Avoid release to the environment.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P370+P378-In case of fire: Use water/water spray/water jet/carbon dioxide/sand/foam/alcoholresistant foam/chemical powder to extinguish.

Supplemental information

• MIXTURE LIST-Contains : tert-Butyl hydroperoxide

For Research Use Only. Not for use in diagnostic procedures.