Gibco™ CHO CD EfficientFeed™ B AGT™ Nutrient Supplement

CHO CD EfficientFeed™ B AGT™ Nutrient Supplement

Product Code	Brand	Quantity
11380892	Gibco™ A1245605	10 L
11370892	Gibco™ A1245601	100 L

Description

Gibco™ CHO CD EfficientFeed™ B AGT™ nutrient supplement is a single part dry format product for fed batch addition to the culture of multiple cell lines. CHO CD EfficientFeed™ B AGT™ (Advanced Granulation Technology™) nutrient supplement is an animal origin-free, chemically defined formulation that contains no proteins, hydrolysates, or components of incompletely defined composition.

The AGT™ format is designed for large scale manufacturing and ease of use. This product is also available in liquid format for small scale use and experimentation.

Expect up to 2- to 5-fold increases in productivity

Avoid issues associated with the use of hydrolysates and other alternatives while gaining superior performance

Simplify scale-up by leveraging the value of AGT™ simple reconstitution

Achieve performance comparable to our original liquid catalog product

Ready for larger scale use with convenient packaging options (10 L and 100 L)

Which EfficientFeed™ Nutrient Supplement to Choose?
Consider the CHO CD Efficient Feed™ Kit when:

You are using a base medium with less than 6 g/L glucose and no hydrolysate at day 0

Consider CHO CD EfficientFeed™ A supplement when:

You are currently using CD OptiCHO™ as your base cell growth medium

Consider CHO CD EfficientFeed™ B supplement when:

You are currently using CD CHO as your base cell growth medium

Consider CD EfficientFeed™ C AGT™ supplement when:

You want higher productivity in GS CHO and other selected cell lines

You are currently using CD FortiCHO™ as your base cell growth medium

You are using a base medium with greater than 6 g/L glucose or a hydrolysate at day 0

Increased Titers
CHO CD EfficientFeed™ supplements have been shown to increase titers 2-5 fold versus batch culture, and are an excellent starting point for process development (Figures 1a & 1b). Furthermore, they outperform competitive alternatives, including hydrolysates (Figures 2, 3a, & 3b).

Simple Scale-Up and Cost Savings
The ability to scale up in a dry format provides ease of handling and storage in manufacturing and other bioprocess settings. With AGT™ (Advanced Granulation Technology™) dry format, it is possible to store and use the product even more easily and safely than traditional dry format products. CHO CD EfficientFeed™ B AGT™ nutrient supplement is offered in 10 L and 100 L packaging that allows scale-up from the 1 L catalog liquid as a seamless process, as well as allow for cost savings when purchasing production level volumes.

Comparable Performance to Liquid Supplement
Equally important as the convenience and simplicity of AGT™ (Advanced Granulation Technology™) dry format is the comparable performance to the liquid CHO CD EfficientFeed™ B (Figure 4). Both forms have performed similarly in our testing, and the AGT™ version has been used successfully as a prototype in many processes by multiple companies.

AGT™ Format Ease of Use
CHO CD EfficientFeed™ B AGT™ nutrient supplement delivers dry format scale-up convenience as well as simple reconstitution via water addition and a single pH adjustment step (see Product Profile Sheet below for complete instructions).

For research or further manufacturing use. Not for use in diagnostic procedures.

Specifications

• Content And Storage: Dry format medium
  Store at 2 to 8°C, Store Dark and Dry
• Classification: Animal Origin-free, Chemically-defined, Protein-free, Serum-free
• Form: AGT
• Serum Level: Serum-Free
• With Additives: Sodium Pyruvate, Sodium Bicarbonate
• Endotoxin Level: Low
• Remarks: Chemically Defined Nutrient Feeding Supplement
• Tested For: Solubility

• Cell Line: CHO
• Culture Type: Suspension Cell Culture
• Product Type: Nutrient Supplement
• Sterility: Non-sterile
• Without Additives: No Glutamine, No Phenol Red, No Insulin
• Purity or Quality Grade: Cell Culture Grade, Qualified for Further Manufacturing, Research Grade
• Shipping Condition: Wet Ice

For Research Use or Further Manufacturing. Not for use in diagnostic procedures.