Invitrogen™ Click-iT™ sDIBO Alkynes for copper-free click chemistry

Experience copper-free 'click chemistry' with Click-IT™ Alexa Fluor™, and sDIBO Alkyne reagents, which are ideal for surface labeling of live cells and minimize damage to enzymes and fluorescent proteins.

Product Code	Brand	Label or Dye	Reactive Moiety	Molecular Weight (g/mol)
10774701	Invitrogen™ C10407	Alexa Fluor™ 594	DIBO Alkyne	∼1200
10598370	Invitrogen™ C10413	Maleimide	DIBO Alkyne, Maleimide	∼440
15848836	Invitrogen™ C20020	Alexa Fluor™ 488	sDIBO Alkyne	∼1400
15930573	Invitrogen™ C20021	Alexa Fluor™ 555	sDIBO Alkyne	∼1800
15940573	Invitrogen™ C20022	Alexa Fluor™ 647	sDIBO Alkyne	∼1900
15950573	Invitrogen™ C20023	Biotin	sDIBO Alkyne	∼1000
15970573	Invitrogen™ C20025	SDP ester	SDP ester, sDIBO Alkyne	∼1050

Description

Achieve efficient labeling of azides with Click-It sDIBO Alkynes. Our Click-IT sDIBO alkynes enable the labeling of azido labeled targets without the need for copper catalysis and are available with a variety of Alexa Fluor or biotin labels, as well as amine or thiol reactive groups for creating your own conjugates. Macromolecules that have been azido-modified enzymatically, chemically, or metabolically can now be labeled easily, yielding soluble bioconjugates.

Click-iT sDIBO Alkyne labels react with azides via a copper-free Click chemistry reaction to form a stable triazole linkage with an Alexa Fluor, Biotin, or label of your choice. Copper-free click reactions using our sDIBO alkynes are ideal for surface labeling of live cells; they also minimize damage to fluorescent proteins like GFP or R-PE. For intracellular labeling, the nonspecific binding of DIBO alkynes creates a background signal, and a labeling strategy using picolyl azides is the preferred solution in these instances.

Click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background. Macromolecules that have been azide-modified enzymatically, chemically, or metabolically can be labeled more easily, yielding more soluble bioconjugates with improved biological labeling utility.

To make a concentrated stock solution from 2–5 mM, dissolve the dye-alkyne in high quality anhydrous DMSO. Store frozen, desiccated, and protected from light. Click-iT SDP Ester sDIBO alkyne is susceptible to hydrolysis, so prepare the stock solution fresh on the day of the experiment.

Specifications

• Green Features: Less hazardous
• Labeling Method: Click Chemistry
• Shipping Condition: Dry Ice
• Labeling Target: Azide