Invitrogen™ Click-iT™ TUNEL Alexa Fluor Imaging Assays for Microscopy & HCS

Screen apoptotic cells with Click-iT TUNEL Assay kits, which offer easy Alexa Fluor dye incorporation and can be multiplexed with GFP and RFP.

Product Code	Brand	Color	Label or Dye	No. of Reactions
10024014	Invitrogen™ C10246	Red	Alexa Fluor™ 594, Hoechst 33342	1 X 96 tests or 50 coverslips
10338924	Invitrogen™ C10247	Far-Red	Alexa Fluor™ 647, Hoechst 33342	1 X 96 tests or 50 coverslips
10798585	Invitrogen™ C10245	Green	Alexa Fluor™ 488, Hoechst 33342	1 X 96 tests or 50 coverslips

Description

Screen apoptotic cells easily and efficiently in just two hours with the Click-iT TUNEL Alexa Fluor 488, 594, and 647 imaging assays. Intended for use with microscopy and high-content screening (HCS), these TUNEL assay kits use a copper-catalyzed reaction to detect incorporated alkyne-modified dUTPs on terminal DNA ends of fragmented DNA. Compared with assays using other modified nucleotides, these apoptosis detection kits are fast (complete within two hours) and can detect a higher percentage of apoptotic cells under identical conditions. Click-iT TUNEL assays also allow multiplexing with surface and intracellular biomarker detection methods, including those that measure fluorescent signal from GFP or RFP.

Apoptosis is marked by defined cellular processes, including cell rounding, blebbing, and DNA fragmentation. The TUNEL assay is the most widely used analytical method to detect fragmented DNA in apoptotic cells in tissue samples, beginning with the incorporation of modified dUTPs at the 3’-OH ends of fragmented DNA. The dUTPs often include a fluorophore molecule. Due to the size of the fluorophore, modified dUTPs can display lower than expected incorporation rates, negatively affecting the sensitivity of the TUNEL assay. Quite often, fluorophores used in currently available TUNEL assay kits exhibit photobleaching and fluorescent spectral overlap issues, both of which reduce the sensitivity of and ability to multiplex these assays.

The Click-iT Plus TUNEL assay was developed to address these issues and is based on a copper (I) catalyzed (i.e., click) reaction between an azide and alkyne. The small size of the Alexa Fluor azide, which has a molecular weight of about 1 kDa, enables easier incorporation of the Alexa Fluor 488, 594, or 647 dye into complex samples, as compared to larger (MW ˜100,000 Da) antibodies. This permits mild fixation or permeabilization and faster assay turnaround time (about two hours) and enables the detection of a higher percentage of apoptotic cells under identical conditions. Because of its gentle reaction conditions, the Click-iT Plus TUNEL assay can also be multiplexed with fluorescent proteins or dyes.

The Click-iT TUNEL Alexa Fluor imaging assays contain all components needed to accurately and reliably detect apoptosis in adherent cells grown on coverslips or 96-well microplates, and they also include DNase I to generate strand breaks for the positive control.

Specifications

• Format: 96-well Plate
• No. of Reactions: 1 X 96 tests or 50 coverslips
• Product Line: Click-iT
• For Use With (Equipment): Fluorescence Microscope, High Content Instrument

• Product Type: Imaging Assay
• Shipping Condition: Dry Ice
• Detection Method: Fluorescence
• Label Type: Alexa Fluor™ Dyes, Classic Dyes