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Invitrogen™ Complement C5a Human ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ BMS2088
300.68 GBP valid until 2024-09-28
Use promo code "21626" to get your promotional price.
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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”21626” to get your promotional price
Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human C5a
Description
The Human Complement C5a (Hu C5a) ELISA quantitates Hu C5a in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu C5a. Principle of the method The Human C5a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
C5 is part of the membrane attack complex (MAC) of the complement system. C5 is split into C5a og C5b by the C5 convertases from the classical and the alternative complement pathway and the C5b fragment initiates the assembly of the terminal MAC (C5b-C9). The molecular mass of C5 is 190 kDa.Specifications
P01031 | |
0.005 ng/mL | |
ELISA Kit | |
Human | |
Colorimetric Microplate Reader | |
C5Da,C5b,CPAMD4,ECLZB,C5 | |
3.6% | |
HRP | |
RUO | |
2°C to 8°C | |
Human | |
4 hr. 30 min. |
0.078-5.0 ng/mL | |
Biotin | |
Plasma, Serum, Supernatant | |
ELISA | |
727 | |
4% | |
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
96 Tests | |
Plasma, 10 μL; Serum, 5 μL; Supernatant, 50 μL | |
complement component 5, C5a, C5D, C5b, ECLZB, CPAMD4 | |
1 hr. 20 min. |