Corning™ cellgro™ Basal Cell Culture Liquid Media - DMEM and Ham's F-12, 50/50 Mix

Used for culture of epithelial, endothelial, and granulosa cell types under low serum conditions. Corning™ cellgro™ Basal Cell Culture Liquid Media - DMEM and Ham's F-12, 50/50 Mix has low levels of endotoxin, and absence of contamination.

Brand:  Corning™ 10-092-CV

Product Code. 15383541

Description

• Modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME
• Fifty-fifty mixture of DMEM and F-12, originally formulated for rat neuroblastoma cells and MDCK cells
• Serum-free growth may be possible with appropriate protein supplements
• Ham's F-12 media is based on Ham's F-10 medium with increased concentrations of choline, inositol, putrescine, and several amino acids
• Formulation of DMEM/Ham's F-12 contains L-glutamine
• Sterile

Specifications

• Content And Storage: 2° to 8°C
• Form: Liquid
• Sterility: Sterile
• Endotoxin Level: <0.25EU/mL
• For Use With (Application): To assess growth promotion and cytotoxicity characteristics
• Quantity: 6 x 500 mL

• Concentration: 1 X
• Product Type: DMEM and Ham's F-12, 50/50 Mix
• With Additives: Glutamine, HEPES
• Formulation: Inorganic Salts: 116.65mg/L CaCl2 (anhydrous), 0.417mg/L FeSO4.7H2O, 0.05mg/L Fe(NO3)3.9H2O, 311.8mg/L KCl, 0.0008mg/L CuSO4 (anhydrous), 84.95mg/L MgSO4 (anhydrous), 7000mg/L NaCl, 62.5mg/L Na2HPO4.H2O, 71mg/L Na2HPO4 (anhydrous), 2438mg/L NaHCO3, 0.4315mg/L ZnSO4.7H2O; Amino Acids: 4.45mg/L L-Alanine, 147.5mg/L L-Arginine. HCl, 7.5mg/L L-Asparagine.H2O, 6.65mg/L L-Aspartic acid, 17.56mg/L L-Cysteine.HCl.H2O, 31.285mg/L L-Cysteine.2HCl, 7.35mg/L L-Glutamic acid, 365.1mg/L L-glutamine, 18.75mg/L Glycine, 31.48mg/L L-Histidine.HCl.H2O, 54.37mg/L L-Isoleucine, 58.95mg/L L-Leucine, 91.35mg/L L-Lysine. HCl, 17.24mg/L L-Methionine, 35.48mg/L L-Phenylalanine, 17.25mg/L L-Proline, 26.25mg/L L-Serine, 53.55mg/L L-Threonine, 9.02mg/L L-Tryptophan, 55.815mg/L L-Tyrosine.2Na.2H2O, 52.85mg/L L-Valine; Vitamins: 0.00365mg/L Biotin, 2.24mg/L D-Ca-Pantothenate, 8.98mg/L Choline chloride, 2.65mg/L Folic acid, 12.61mg/L i-Inositol, 2.0185mg/L Nicotinamide, 2.031mg/L Pyridoxine. HCl, 0.219mg/L Riboflavi
• pH: 7 to 7.4