Thermo Scientific Chemicals Cresol Red

Description

Cresol red is commonly used with lead acetate for the detection of urease after PAGE. It is also used as an indicator with the following transition intervals: pH 1.8 (orange) to pH 2.0 (yellow); pH 7.0 (yellow) to pH 8.8 (violet). Cresol Red is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) electrophoresis. In agarose, Cresol Red runs with an apparent molecular size of approx. 125 bp DNA. Cresol Red does not inhibit Taq polymerase.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Cresol red is commonly used with lead acetate for the detection of urease after PAGE. It is also used as an indicator with the following transition intervals: pH 1.8 (orange) to pH 2.0 (yellow); pH 7.0 (yellow) to pH 8.8 (violet). Cresol Red is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) electrophoresis. In agarose, Cresol Red runs with an apparent molecular size of approx. 125 bp DNA. Cresol Red does not inhibit Taq polymerase.

Solubility
Soluble in water (slightly at 20°C), 50% ethanol (0.1%), dil. acids, and dil. alkalis. Insoluble in benzene.

Notes
Transition interval (acid): pH 1.8 (orange) to pH 2.0 (yellow); (alkaline) pH 7.0 (yellow) to pH 8.8 (violet)

Specifications

Chemical Identifiers

• CAS: 1733-12-6
• Molecular Formula: C21H18O5S
• Molecular Weight (g/mol): 382.43
• MDL Number: MFCD00005878
• InChI Key: OBRMNDMBJQTZHV-UHFFFAOYSA-N
• Synonym: o-Cresolsulfonephthalein
• PubChem CID: 73013
• ChEBI: CHEBI:86218
• IUPAC Name: 4-[3-(4-hydroxy-3-methylphenyl)-1,1-dioxo-2,1$l^{6}-benzoxathiol-3-yl]-2-methylphenol
• SMILES: CC1=CC(=CC=C1O)C1(OS(=O)(=O)C2=CC=CC=C12)C1=CC=C(O)C(C)=C1

• Melting Point: >300°C
• Beilstein: 343399
• Merck Index: 14,2578
• Solubility Information: Soluble in water (slightly at 20°C),50% ethanol (0.1%),dil. acids,and dil. alkalis. Insoluble in benzene.
• Formula Weight: 382.44
• Quantity: 25 g
• Chemical Name or Material: Cresol Red

RUO – Research Use Only