Invitrogen™ CSM Media for Mav203 Yeast Cells

A specifically designed media for making agar plates that enable the growth of Saccharomyces cerevisae MaV203 cells

Brand:  Invitrogen™ A13292

Product Code. 12353593

Description

The CSM Media for Mav203 Yeast Cells is a specifically designed media for making agar plates that enable the growth of Saccharomyces cerevisae MaV203 cells. This product comes with enough reagents to prepare 2 liters of solid media with the following components per liter:

• CSM (without tryptophan): 0.74g
• Yeast Nitrogen Base: 6.66g
• Ammonium Sulfate: 5g
• Glucose: 20g
• Agar: 20g

Compatible with All MaV203 Applications
The CSM Media is optimized for the GeneArt™ High-Order Genetic Assembly System, including the recovery for transformations of vectors once they have been adapted to yeast using the GeneArt™ High-Order Vector Conversion Cassette. The media can also be used to grow MaV203 competent yeast cells, both library and sub-cloning scale (cat# 11281-011 and 11445-012).

Plate Preparation Protocol
For each liter, use one pouch of CSM -Trp Agar Yeast Media minus Glucose and 1 bottle of 20% Glucose. Dissolve the contents of the pouch in 900mL of distilled water and autoclave for 20 minutes on liquid cycle. Allow the solution to cool to 55°C and then add one bottle of 20% Glucose (100mL). Mix well and pour plates.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

• Content And Storage: CSM Media for MaV203 Yeast Cells has enough reagents to prepare 2 liters of agar media. The product contains two pouches of premixed powder and two 100 ml bottles of filter-sterilized, 20% glucose.
  
  Store all components at room temperature.
  
  All components are guaranteed stable for 6 months when properly stored.
• Media Type: CSM Media
• Product Type: Microbiology Media
• Target Organism Class: S. cerevisiae
• For Use With (Application): Optimized for use with the GENEART™ High-Order Genetic Assembly System, A13285
• Quantity: 2 L
• Strain Designations: MaV203

• Form: Liquid, Powder
• Preparation Method: Autoclave
• Sterility: Non-sterile
• Final Product Type: Agar Plates
• Product Line: GeneArt™
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.