Gibco™ ExpiCHO™ Expression Medium

A GMP-manufactured, chemically-defined, serum-free, protein-free, animal origin–free medium specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells

Product Code	Brand	Quantity
15421783	Gibco™ A2910001	1000 mL
15492163	Gibco™ A2910002	6 x 1 L
15471044	Gibco™ A2910004	20 L
15461044	Gibco™ A2910003	10 L

Description

ExpiCHO Expression Medium is specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells. This medium is GMP-manufactured, chemically-defined, serum-free, protein-free, and animal origin–free. It is a core component of the ExpiCHO Expression System Kit (Cat. No. A29133) for scalable transient protein production. To achieve the highest levels of transient protein expression, the medium is matched to a culture feed, ExpiCHO Feed, also included in the system kit and transfection kits.

With ExpiCHO Expression Medium you can:
• Grow suspension CHO cultures to densities of over 2 x 10⁷ cells per mL, with >95% cell viability
• Achieve high transfection efficiencies of >90% when coupled with ExpiFectamine CHO transfection reagent
• Transiently transfect suspension CHO cells at a density of 6 x 10⁶ cells per mL
• Achieve yields up to 3 g per L of recombinant protein of high-density transiently transfected cultures by sustained, high-level expression
• Grow and transfect CHO cells at volumes of <1 mL up to 5 L in culture formats from multiwell plates up to 5 L shake flask

ExpiCHO Expression Medium is ready to use.  It is formulated with GlutaMAX-I reagent and does not require supplementation. It does not contain phenol red. The chemically-defined, animal origin free formulation results in high reproducibility and lot-to-lot reliability. ExpiCHO Expression Medium is not recommended for adherent cell cultures.

Specifications

• Content And Storage: Storage conditions: in the dark at 2-8°C
  Shipping conditions: ambient
  Shelf life: 12 months from date of manufacture
• Classification: Animal Origin-free, Chemically-defined, Protein-free, Serum-free
• Form: Liquid
• Serum Level: Serum-free
• Without Additives: No Phenol Red
• Shipping Condition: Room Temperature

• Cell Line: ExpiCHO-S™, CHO-S, SFM Adapted
• Culture Type: Suspension Cell Culture
• Product Type: CHO Expression Medium
• With Additives: GlutaMAX
• Product Line: ExpiCHO™