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CXCL1 Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

£171.00 - £1422.00

Specifications

Antigen CXCL1
Concentration 0.1-1.0 mg/mL
Applications ELISA, Western Blot
Classification Polyclonal
Conjugate Unconjugated
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Products 3
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
17833673
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Invitrogen
500-P74-50UG
50 μg
£171.00
50µg
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17833213
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Invitrogen
500-P74-100UG
100 μg
£220.00
100µg
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17803273
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Invitrogen
500-P74-1MG
1 mg
£1422.00
1mg
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Description

Description

AA Sequence of recombinant protein: APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA CLDPEAPMVQ KIVQKMLKGV PK. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat GRO/KC (CXCL1). Anti-Rat GRO/KC (CXCL1)-specific antibody was purified by affinity chromatography employing an immobilized Rat GRO/KC (CXCL1) matrix. Sandwich ELISA: To detect Rat GRO/KC (CXCL1) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat GRO/KC (CXCL1) (500-P74Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat GRO/KC (CXCL1). Western Blot: To detect Rat GRO/KC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat GRO/KC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
Specifications
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