Invitrogen™ D-Dimer Human ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15414973	Invitrogen™ EHDDIMER	96 Tests
17884190	Invitrogen™ EHDDIMERX10	10 x 96 Tests
17155419	Invitrogen™ EHDDIMERX5	5 x 96 Tests

Description

The Human D-Dimer ELISA quantitates Hu D-Dimer in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu D-Dimer. Principle of the method The Human D-Dimer solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Fibrinogen is the main protein of blood coagulation system. It consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. The process of blood coagulation results in the activation of fibrinogen into fibrin by thrombin and fibrin polymerization. Fibrin clot is then digested by plasmin, and fibrin degradation products of different molecular weights are released into the bloodstream.

Specifications

• Accession Number: P02671
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Fib2
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Sensitivity: 0.08 pg/mL
• Conjugate: Biotin
• Protein Family: Cell Structure & Organelle Proteins
• Specificity: This ELISA pair antibody detects human D-Dimer. Other species not determined.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 2243
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Extracellular Matrix & Cell Adhesion
• Synonym: Fib2
• Test Time: 1 hr. 20 min.