Applied Biosystems™ dGTP BigDye™ Terminator v1.0 Ready Reaction Cycle Sequencing Kit

dGTP BigDye™ terminator cycle sequencing kit with the AmpliTaq™ DNA polymerase, FS. This ready reaction kit uses the same dye set as the BigDye™ v1.0 kits.

Brand:  Applied Biosystems™ 4307175

Product Code. 15839746

Includes: Contains AmpliTaq™ DNA Polymerase, FS, BigDye terminators v1.0, pGEM™ - 3Zf(+) Control Template, -21 M13 Control Primer (forward), and Premixed Ready Reaction.

Description

• Ensure reliable results with difficult regions and finishing applications
• Sequence templates with challenging sequence contexts
• Sequence problematic templates that standard chemistries cannot
• Optimized for GT-Rich templates: Obtain higher success rate even with your difficult GT-rich templates
• By using the same chemistries as the standard BigDye™ terminator kits (only replacing dITP with dGTP) the dGTP BigDye™ terminator kit provides proven performance
• Optimized for GT-rich and other difficult-to-sequence templates
• Easily Integrate into your workflow: dGTP BigDye™ terminator kits require no new software or instrument recalibration, so you can easily integrate it into your current workflow with minimal changes to your protocols
• The dGTP BigDye™ terminator kit replaces dITP with dGTP and it enables you to extend through those difficult-to-sequence regions with confidence, avoiding early signal loss in these samples

Order Info

100 rxns

Specifications

• Content And Storage: The kit contains the AmpliTaq™ DNA Polymerase, FS, BigDye
  terminators v1.0, pGEM™ - 3Zf(+) Control Template,
  -21 M13 Control Primer (forward), and Premixed Ready Reaction.
  Store kit at -15 to -25°C.
• For Use With (Application): GT-Rich or Difficult Template Sequencing
• Product Line: BigDye™ Terminator, BigDye™
• Technique: Fluorescent Dye Terminator Sequencing

• Format: 12-well Strips, 8-well Strip, 96- or 384-well Plate
• No. of Reactions: 100
• Product Type: Sequencing Kit
• Template Compatibility: BAC DNA, Genomic DNA (Bacterial), Plasmid DNA (≤15Kb), PCR Amplicons, Single Stranded DNA

For Research Use Only. Not for use in diagnostic procedures.