Diethyl Pyrocarbonate, MP Biomedicals™

Description

Used to inactivate RNase enzymes. Diethyl Pyrocarbonate, MP Biomedicals is sensitive to moisture and pH. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen. It protects RNA against degradation and is effective

DEPC inactivates RNase in solution at about 0.1% (v/v). It inhibits the ryanodine binding to ryanodine/Ca2+ receptor channels in skeletal muscle in a dose- and time-dependent manner and increases the Ca2+ permeability of SR vesicles. DEPC is effective as a nuclease inhibitor with a variety of uses:

• Modification reagent for His and Tyr residues in proteins
• Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases
• Used to modify proteins and nucleic acids
• PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases
• Dot blot hybridization, to dilute total RNA isolated from different micro-organisms

Specifications

• CAS: 1609-47-8
• Density: 1.101g/mL at 25°C (Lit.)
• Boiling Point: 93°C to 94°C (18 mmHg)(literature)
• Molecular Formula: C6H10O5
• Refractive Index: n20/D 1.398 (Lit.)
• MDL Number: MFCD00009106
• Quantity: 100 mL
• Synonym: diethyl pyrocarbonate, diethyl dicarbonate, depc, diethyl oxydiformate, baycovin, diethylpyrocarbonate, ethyl pyrocarbonate, dicarbonic acid, diethyl ester, dicarbonic acid diethyl ester, diethyl pyrocarbonic acid
• Solubility Information: Soluble in alcohols, esters, ketones, hydrocarbons. A 50% w/w solution in ethanol may be prepared. Slowly soluble in water with hydrolytic decomposition yielding ethanol and CO2.
• InChI Key: FFYPMLJYZAEMQB-UHFFFAOYSA-N
• SMILES: CCOC(=O)OC(=O)OCC
• IUPAC Name: ethoxycarbonyl ethyl carbonate
• Molecular Weight (g/mol): 162.14
• PubChem CID: 3051
• ChEBI: CHEBI:59051
• Formula Weight: 162.10
• Concentration or Composition (by Analyte or Components): Working Concentration: For Treatment of water to inhibit Rnase: Add 1mL DEPC to 1L water. Let sit overnight. Autoclave.
• Physical Form: Liquid

Safety and Handling

Product Identifier

• Diethyl Pyrocarbonate

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.

Precautionary Statement

• P101-If medical advice is needed, have product container or label at hand.
• P102-Keep out of reach of children.
• P103-Read label before use.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : diethyl pyrocarbonate