Thermo Scientific Chemicals DL-1,4-Dithiothreitol, 99%, for biochemistry

Description

1,4-Dithiothreitol, CAS # 3483-12-3, is a reducing agent commonly used to reduce disulphide bonds in protein studies, such as western blot SDS-page electrophoresis.

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General description

• 1,4-Dithiothreitol (DTT or Cleland's reagent) reduces disulfide bonds in proteins, leading to loss of native conformation
• DTT is not stable in solution and should be prepared in small aliquots prior to use; the use of EDTA in the solution can increase the half-life of DTT
• Typically used at a final concentration of 0.1M for protein stabilization; recommended to create a 1M stock

Applications

• Protects thiol groups from oxidation and acts as a chemical chelator in solution; maintains proteins in the denatured state
• Common applications include SDS-page electrophoresis and protein isolation

Specifications

Chemical Identifiers

• CAS: 3483-12-03
• MDL Number: MFCD00004877
• InChI Key: VHJLVAABSRFDPM-IMJSIDKUSA-N
• Synonym: dithiothreitol, dl-1,4-dithiothreitol, dl-dithiothreitol, 1,4-dithio-dl-threitol, d-1,4-dithiothreitol, d-dtt, 2s,3s-1,4-dimercaptobutane-2,3-diol, threo-1,4-dimercapto-2,3-butanediol, 1,4-dithiothreitol, dtt
• PubChem CID: 446094
• ChEBI: CHEBI:42170
• IUPAC Name: (2S,3S)-1,4-bis(sulfanyl)butane-2,3-diol
• SMILES: C(C(C(CS)O)O)S

• Absorbance: (1M soln. in water), (1 M soln. in water) at 0nm, 0.035 max. at 500nm
• Melting Point: 39.0°C to 44.0°C
• Color: White
• Boiling Point: 125.0°C to 130.0°C (2.0 mmHg)
• Flash Point: >110°C
• Infrared Spectrum: Authentic
• Assay Percent Range: 98.5% min. (Iodimetry)
• Molecular Formula: C₄H₁₀O₂S₂
• Linear Formula: HSCH₂CH(OH)CH(OH)CH₂SH
• Quantity: 500 g
• Beilstein: 01, III, 2360
• Merck Index: 15, 3419
• Solubility Information: Solubility in water: freely soluble. Other solubilities: freely soluble in ethanol,acetone,ether,,chloroform and ethyl acetate
• Transmittance: 99% min. (c=5, 660nm, water) 1cm cell
• Molecular Weight (g/mol): 154.25
• Formula Weight: 154.25
• Percent Purity: 99%
• Grade: Biochemistry
• Physical Form: Powder
• Chemical Name or Material: DL-1, 4-Dithiothreitol

Safety and Handling

Product Identifier

• DL-1,4-Dithiothreitol

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.
• H302-Harmful if swallowed.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P302+P352-IF ON SKIN: Wash with plenty of water/.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.

RUO – Research Use Only