Gibco™ DMEM/F-12, HEPES, powder

DMEM/F-12, HEPES, powder

Brand:  Gibco™ 42400010

Product Code. 10655373

Description

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool. Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco™ DMEM / F-12 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco™ DMEM / F-12 product made in our Grand Island facility (12400-024). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Specifications

• Certifications/Compliance: ISO 13485 standards, FDA
• Description: Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM / F-12), 1L, Powder, 5.6 to 6.0 pH, With L-Glutamine, HEPES, Phenol Red, Sodium Pyruvate, 36 Months Shelf Life, 260 to 300mOsm/kg Osmolality, For Mammalian Cell Culture
• Product Type: Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12
• Without Additives: No Sodium Bicarbonate
• pH: 5.6 to 6.0

• Content And Storage: 2 to 8°C
• Form: Powder
• With Additives: Glutamine, HEPES, Phenol Red, Sodium Pyruvate
• For Use With (Application): Mammalian cell culture
• Quantity: 1 L