Gibco™ DMEM/F-12, no phenol red

DMEM/F-12, no phenol red

Brand:  Gibco™ 21041033

Product Code. 12088089

Description

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

cGMP manufacturing and quality system
For supply chain continuity, we manufacture DMEM/F-12 at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

Specifications

• Certifications/Compliance: ISO 13485 standards, FDA
• Description: DMEM Medium, 500mL, 1 X Concentration, Liquid Form, With L-glutamine, Sodium Pyruvate, 7 pH, 12 Months Shelf Life, 2 to 8°C Storage Conditions, Soluble in Water, Used for Mammalian Cell Culture
• Form: Liquid
• With Additives: Glutamine, Sodium Pyruvate
• For Use With (Application): Mammalian cell culture
• Quantity: 10 x 500 mL

• Content And Storage: 2 to 8°C
• Concentration: 1 X
• Product Type: DMEM Medium
• Without Additives: No HEPES, No Phenol Red
• pH: 7