Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment

DNA Polymerase I, Large (Klenow) Fragment

Brand:  Invitrogen™ 18012096

Product Code. 10023042

Description

DNA Polymerase I, Large (Klenow) Fragment is a DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities.

Applications:
Fill-in of 5´ overhangs (1). Synthesis of probes by random primers labeling method (2). Sequencing single- and double-stranded DNA (3). Site-directed mutagenesis.

Source:
Purified from E. coli expressing the Klenow fragment on a plasmid.

Performance and Quality Testing:
SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.

Unit Definition:
One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template

primer.

Specifications

• Content And Storage: –20°C
• Quantity: 2 x 500 Unit(s)
• Includes: DNA polymerase I, large (Klenow) fragment is supplied with a vial of 10X REact™ 2 buffer [500mM Tris HCl (pH 8.0), 100mM MgCl2, 500mM NaCl], vial of dilution buffer

• Description: DNA Polymerase I Large (Klenow) Fragment
• For Use With (Application): Genotyping, Cloning

For Research Use Only. Not for use in diagnostic procedures.