Thermo Scientific™ DRAQ5™ Fluorescent Probe Solution (5 mM)

Stain live or fixed cells with this far-red DNA stain.

Brand:  Thermo Scientific™ 62251

Product Code. 11863865

Description

Stain live or fixed cells with Thermo Scientific™ DRAQ5 Fluorescent Probe, a far-red DNA stain.

Fluorescence cell-based assays, such as flow cytometry, in-cell ELISA, fluorescence microscopy and high-content imaging require a fluorescent label to identify individual cells. When using multiple fluorescent probes to detect different cellular targets or activities, each probe must have a fluorescent spectrum different than the other probes. The blue-fluorescent DNA-binding probes, Hoechst and DAPI are frequently used; however, these probes cannot be used when UV illumination is unavailable or other blue-emitting fluorescent probes are used. Therefore, nuclear probes that emit in a color other than blue is useful for cell identification and counting, and for determining nuclear morphology and DNA content.

• Due to its far-red excitation and emission, the DRAQ5 Fluorescent Probe can be multiplexed with many other fluorophores and is ideal for cells expressing green fluorescent protein (GFP) fusion proteins
• Compatible with many existing protocols across a wide range of instrumentation platforms
• Emits in the far-red region, is lipophilic and crosses cell and nuclear membranes in live and fixed cells and tissues for rapid DNA staining
• Stain is water-soluble, supplied ready-to-use and does not require RNase, cell lysis, or a washing step, making it compatible with automation
• Because DNA staining is stoichiometric, the DRAQ5 Fluorescent Probe can be used for DNA content analysis in cell proliferation studies

Spécification

• Cell Permeability: Cell-permeant
• Content And Storage: Store at 4°C.
• Detection Method: Fluorescence
• For Use With (Equipment): Fluorescence Microscope, High Content Analysis Instrument
• Dye Type: Cell-Permeant
• Excitation Wavelength Range: 647 nm
• Product Line: Pierce™
• Quantity: 200 μL
• Sub Cellular Localization: Nucleus, Cytosol, Cytoplasm

• Color: Red
• Description: DRAQ5™ Fluorescent Probe Solution (5 mM)
• For Use With (Application): Cell Imaging
• Product Type: Fluorescent Probe Solution
• Emission: 681 nm
• Form: Liquid
• Purity or Quality Grade: > 99%
• Solubility: Soluble at working concentrations in water and typical buffers

Sécurité et traitement

Identifiant produit

• DRAQ5 Fluorescent Probe Solution (5 mM)

Mention d'avertissement

• Warning

Catégorie de danger

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Mention de danger

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.

Conseils de prudence

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Informations supplémentaires

• MIXTURE LIST-Contains : 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione

For Research Use Only. Not for use in diagnostic procedures.