Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase

Thermo Scientific DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.

Product Code	Brand	Color	Quantity
15699364	Thermo Scientific™ EP1701	Colorless	200 U
15617423	Thermo Scientific™ EP1701	Colorless	200 U
15609374	Thermo Scientific™ EP1702	Colorless	500 U
15616154	Thermo Scientific™ EP1702	Colorless	500 U
15787496	Thermo Scientific™ EP1702	Colorless	500 U
15619374	Thermo Scientific™ EP1703	Colorless	2500 U
15797496	Thermo Scientific™ EP1703	Colorless	2500 U
15626154	Thermo Scientific™ EP1703	Colorless	2500 U
15636154	Thermo Scientific™ EP1704	Colorless	4 x 2500 U
15629374	Thermo Scientific™ EP1711	Green	200 U
15639374	Thermo Scientific™ EP1712	Green	500 U
15707506	Thermo Scientific™ EP1712	Green	500 U
15649374	Thermo Scientific™ EP1713	Green	2500 U
15666154	Thermo Scientific™ EP1713	Green	2500 U
15605014	Thermo Scientific™ EP1713	Green	2500 U
15659374	Thermo Scientific™ EP1714	Green	4 x 2500 U

Description

Thermo Scientific DreamTaq Hot Start DNA Polymerase, available in colorless and green formats, is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.

DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step. With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases. The enzyme is supplied with an optimized 10X DreamTaq Buffer containing magnesium chloride, which eliminates the need for extensive optimization of reaction conditions. DreamTaq Hot Start DNA Polymerase is also available with pre-added density reagent and electrophoresis tracking dyes, as well as in master mix format.

The 10X DreamTaq Green Buffer also includes a density reagent and two tracking dyes for convenient direct loading of PCR products on a gel.

DreamTaq Hot Start DNA Polymerase is formulated to enhance productivity through:

• Better reaction outcomes
  • Higher yield of target amplicons from low template amounts
  • Increased reaction specificity due to reliable hot-start technology
  • Wider range of amplicon lengths with routine amplification of genomic DNA fragments up to 6 kb
• Enhanced convenience
  • Room temperature reaction setup
  • Minimized optimization of Mg²⁺ concentration and of primer annealing temperatures due to optimized reaction buffer
  • Direct loading of PCR products on a gel with DreamTaq Green Buffer

Applications

• Generation of PCR products for TA cloning
• Routine PCR
• Colony PCR
• Genotyping
• RT-PCR

Specifications

• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Concentration: 5 U/μL
• Overhang: 3'-A

• Polymerase: DreamTaq DNA Polymerase
• For Use With (Application): Hot-start PCR
• Hot Start: Built-In Hot Start
• Reaction Format: Separate Components