DreamTaq DreamTaq™ Hot Start Green PCR Master Mix

Thermo Scientific DreamTaq Hot Start DNA Polymerase is the product of choice when you need consistently robust amplification with minimal optimization steps

Brand:  DreamTaq K9022

Product Code. 15699374

Includes: DreamTaq Hot Start Green PCR Master Mix, 2X; water, nuclease-free

Description

Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that ensures higher PCR specificity, sensitivity and yields compared to conventional hot start Taq DNA polymerases. DreamTaq Hot Start DNA Polymerase uses antibody based inhibition of DNA polymerase activity at ambient temperatures, thus preventing the amplification of non-specific products prior to the amplification step.

Better reaction outcomes: 

• Higher yield of target amplicons from low template amounts.
• Increased reaction specificity due to reliable hot-start technology.
• Wider range of amplicon lengths - routine amplification of genomic DNA fragments up to 6kb.

Enhanced convenience:

• Room temperature reaction set-up.
• Minimized optimization of Mg2+ concentration and of primer annealing temperatures due to optimized reaction buffer.  
• Simplified workflow and minimized pipetting steps with 2X master mix format.  

Amplicon size (complex DNA): 6 kb
Fidelity (vs. Taq): 1X

Recommended Storage

Store at -20C.

Specifications

• Content And Storage: Contains:
  • 20 x 1.25 mL DreamTaq Hot Start Green PCR Master Mix (2X)
  • 20 x 1.25 mL Nuclease-free water
  
  Store at -5 to -30°C.
• Polymerase: DreamTaq DNA Polymerase
• Shipping Condition: Wet or Dry Ice
• Overhang: 3'-A

• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Hot Start: Built-In Hot Start
• Reaction Format: SuperMix or Master Mix