DYKDDDDK Tag Polyclonal Antibody, Agarose, Invitrogen™

Goat Polyclonal Antibody

Brand:  Invitrogen PA132374

Product Code. 11532412

Description

PA1-32374 detects ECS from human samples. PA1-32374 has been successfully used in immunoprecipitation applications. The PA1-32374 immunogen is derived from goats that were immunized with ECS enterokinase cleavage site (xxxDDDDK). Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Goat anti-human ECS affinity purified antibodies were coupled to agarose beads using cyanogens bromide method.

DYKDDDDK Tag (FLAG-tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombit DNA technology. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

Specifications

• Antigen: DYKDDDDK Tag
• Classification: Polyclonal
• Gene Alias: DDDDK; DDDDK epitope tag; DYKDDDDK epitope tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG Tag
• Immunogen: Goats were immunized with ECS enterokinase cleavage site (xxxDDDDK). Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Goat anti-human ECS affinity purified antibodies were coupled to agarose beads using cyanogens bromide method .
• Quantity: 100 μg
• Primary or Secondary: Primary
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunoprecipitation
• Conjugate: Agarose
• Host Species: Goat
• Purification Method: Purified
• Regulatory Status: RUO
• Content And Storage: 4° C
• Form: Slurry
• Target Molecule: Tag