Invitrogen™ Dynabeads™ M-450 Tosylactivated

Dynabeads™ M-450 Tosylactivated

Brand:  Invitrogen™ 14013

Product Code. 15424693

Description

Dynabeads™ M-450 Tosylactivated are 4.5μm superparamagnetic beads containing surface tosyl groups. The beads covalently bind primary amino and sulfhydryl groups in antibodies to position them in the optimal orientation for cell-surface protein binding. Low background and covalent binding of the antibodies make these beads an excellent choice for cell separation applications when a downstream release from the beads is not required.

Quickly deplete cells or positively isolate target cells for downstream molecular analysis

Excellent for cell isolation procedures using your own unique antibody

Several antibodies can be coupled on the same beads for non-cell-based applications, such as cell activation

Coupling procedure
Covalent coupling is performed overnight by incubating the antibody of choice with the Dynabeads™ M-450 Tosylactivated beads. Optimal coupling occurs at high pH (8.5–9.5) and at 37°C. For pH-labile antibodies, coupling can be performed in an alternative buffer at pH 7.4.

Not recommended for immunoprecipitation (IP)
These 4.5μm Tosylactivated beads are not recommended for IP as they have a lower surface capacity per unit mass when compared to 1μm and 2.8μm Tosylactivated and Epoxy Dynabeads™.

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Specifications

• Content And Storage: 2°C to 8°C
• Description: Dynabeads™ M-450 Tosylactivated, 5mL, Suspension Format, Blood, PBMC, Tissue Digests Sample Type, Depletion, Positive Isolation Method, Used For Cell Isolation and Expansion, Protein Purification & Isolation
• Format: Suspension
• No. of Cells: 2 x 10⁷
• Quantity: 5 mL

• Contents: 5mL Beads at a concentration of 4 x 10^8 beads/mL in distilled water
• For Use With (Application): Protein Biology, Protein Purification and Isolation, Cell Lysis and Fractionation, Cell Analysis, Cell Expansion, Cell Isolation, Organelle Isolation
• Includes: 5mL beads at a concentration of 4 x 10^8 beads/mL in distilled water
• Purity or Quality Grade: Research Grade
• Sample Type: Blood, PBMC, tissue digests

For Research Use Only. Not for use in diagnostic procedures.