Invitrogen™ Dynabeads™ RNA Purification Kit

The Dynabeads RNA Purification Kit contains Dynabeads RNA Purification Buffer and Dynabeads Carboxylic Acid for RNA Purification, providing a simple, flexible, and automation-ready solution for purification of crude RNA produced by in vitro transcription (IVT).

Product Code	Brand	Description
30056333	Invitrogen™ 65030D	Small Combo Kit
30055080	Invitrogen™ 65032D	Large Combo Kit

Description

The Dynabeads RNA Purification Kit contains Dynabeads RNA Purification Buffer and Dynabeads Carboxylic Acid for RNA Purification beads, providing a simple, flexible, and automation-ready solution for purification of crude RNA produced by in vitro transcription (IVT). It is particularly suitable where high purity and scalability are required, such as in the discovery phase of RNA-based vaccine and therapeutic development.

Features of the Dynabeads RNA Purification Kit include:
• Efficiency—reuse at least six times to help reduce the cost and time in a simple workflow
• Scalability—swap between manual and automated workflows to match required scale (micrograms to grams of mRNA) and throughput
• Quality—ensure a safe and flexible supply of products to navigate from RNA-based drug discovery research to commercial manufacturing with counterpart solutions (available via the OEM commercial supply), without incurring extra time or costs from switching technology or raw material

Overview of the workflow
Crude RNA from the IVT step is mixed with the Dynabeads Carboxylic Acid for RNA Purification and Dynabeads RNA Binding Buffer is added. The RNA binds to the bead surface and the remaining components of the reaction mixture are removed by applying a magnet (such as thermofisher.com/magnets) and discarding the supernatant (Figure 1).

The products offered in this combo kit can also be purchased separately. In addition, they are included in the Dynabeads In Vitro Transcription and RNA Purification combo kit (Cat. No. 65034D) together with Dynabeads Streptavidin for In Vitro Transcription.

Automation-ready for use with KingFisher purification systems
The 1-μm magnetic beads are ideal for high-throughput screening. The process can be automated using liquid handlers or any of our KingFisher sample purification systems. The scripts for KingFisher Flex and Apex automated RNA purification workflows are available for download from our website.

Commercial supply
To learn more about counterpart products and support documentation available for GMP-compliant manufacturing of mRNA vaccines and therapeutics, visit thermofisher.com/dynabeadsmrna

Specifications

• Isolation Technology: Magnetic Bead
• Sample Type: Crude RNA
• For Use With (Equipment): KingFisher™ Sample Purification System, DynaMag™ magnets
• Product Line: Dynabeads™
• Shipping Condition: Ambient Temperature

• Purification Time: Variable
• High-throughput Compatibility: Yes
• For Use With (Application): In Vitro Transcription, Synthetic RNA, Crude RNA Purification (from IVT Reaction Mix)
• RNase: RNase-free (No detectable activity level)
• Sterility: Non-sterile