Invitrogen™ E-Gel™ CloneWell™ II Agarose Gels with SYBR Safe, 0.8%

Faster and more effective DNA fragment isolation method for successful cloning workflow. Recover your DNA in 3 easy steps.

Brand:  Invitrogen™ G661818

Product Code. 15796548

Includes: Includes 10 gels, and 1 vial 10X Sample Loading Buffer

Description

E-Gel CloneWell II Agarose Gels with SYBR Safe enable faster and more effective DNA fragment isolation for cloning workflows. The use of in-gel SYBR Safe stain and a blue-light transilluminator minimizes DNA damage and improves cloning efficiency compared to conventional methods. The E-Gel CloneWell II agarose gels are designed for use with the E-Gel Power Snap Electrophoresis Device (Cat. No. G8100).

E-Gel CloneWell II agarose gels enable you to:

• Save time—no additional gel purification steps required after electrophoresis
• Simplify DNA recovery—retrieve purified DNA directly from the recovery well with a pipette
• Improve cloning results—minimize DNA damage and get more colony forming units for any cloning method

Fast DNA sample isolation in three steps
DNA fragment isolation with E-Gel CloneWell II precast gels is a simple 3-step procedure that eliminates conventional gel purification. To extract the DNA band of interest, load the sample into the loading wells, run the gel until the band reaches the recovery wells, and retrieve the sample. The covered sample is ready for cloning and does not require re-purification. Multiple bands from the one loading well can be collected during the same gel run.

DNA extracted from CloneWell II gels is compatible with common cloning methods such as Anza restriction cloning, TOPO cloning, and Gateway cloning.

Real-time control of the sample
SYBR Safe DNA Gel Stain is incorporated into the gel itself, allowing you to visually monitor DNA band migration progress on the blue-light transilluminator of the E-Gel Power Snap Electrophoresis Device. The gel has reference marks for better control of the sample prior to the sample recovery step. If the target DNA passes the recovery well, the Reverse gel protocol allows for re-capture of the DNA band.

Improved cloning efficiencies
Even a short exposure of your DNA sample to UV light during visualization may lead to DNA damage and reduced cloning efficiencies. Using E-Gel CloneWell II gels together with the blue-light transilluminator minimizes UV damage and improves cloning efficiency when compared to conventional methods.

Safer alternative to ethidium bromide
The E-Gel CloneWell II agarose gel is pre-stained with SYBR Safe DNA stain that minimizes environmental impact and is safer than the ethidium bromide used in conventional preparative gel electrophoresis.

Specifications

• Compatible Ladders: E-Gel™ 1 Kb Plus Express DNA Ladder
• Gel Percentage: 0.8%
• Product Type: Agarose Gel
• Wells: 7-well
• Shipping Condition: Room Temperature

• Content And Storage: Store at room temperature.
• Gel Type: E-Gel
• Stain Type: SYBR™ Safe
• Green Features: Less hazardous

Safety and Handling

Signal Word

• Warning

Hazard Category

• Skin sensitiser Category 1

Hazard Statement

• H317-May cause an allergic skin reaction.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P272-Contaminated work clothing should not be allowed out of the workplace.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/.
• P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.

For Research Use Only. Not for use in diagnostic procedures.