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STEMCELL Technologies EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit
Immunomagnetic negative selection of human CD4+CD127lowCD25+ regulatory T cells
Brand: STEMCELL Technologies 18063
This item is not returnable.
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Description
- This kit isolates human CD4+CD127lowCD25+ regulatory T cells (Tregs) from fresh peripheral blood mononuclear cells (PBMCs) or leukapheresis samples
- First, CD25+ cells are isolated by column-free immunomagnetic positive selection using EasySep™ Releasable RapidSpheres™
- Then, bound magnetic particles are removed from the EasySep™-isolated CD25+ cells, and unwanted non-Tregs are targeted for depletion
- The final isolated fraction contains highly purified CD4+CD127lowCD25+ cells that express high levels of FOXP3 and are immediately ready for downstream applications
- An optional protocol allows for the isolation of CD4+CD25- responder T cells in parallel for use in functional studies
- Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands
Specifications
T Cells, T Cells, CD4+, T Cells, Regulatory | |
Cell Isolation | |
1 x 109 cells |