Merck Scepter 2.0 Cytometer Sensors

Designed for use with the Scepter 2.0 Automated Handheld Cell Counter (cat. no. PHCC20040). Scepter 2.0 Cytometer Sensors ensure accurate, reproducible counts and eliminate pipetting errors and uneven sample loading. Available in two apertures to count cells between 4 and 25 μm.

Product Code	Brand	Aperture Size	Cell Size	Particle Size	Operating Cycles
11502134	Merck PHCC40050	40μm	4 to 12μm	3 to 18μm	50,000 to 1,500,000 particles/mL
11522134	Merck PHCC60050	60μm	8 to 25μm	6 to 36μm	10,000 to 500,000 particles/mL
11604133	Merck PHCC40500	40μm	4 to 12μm	3 to 18μm	50,000 to 1,500,000 particles/mL
11634133	Merck PHCC60500	60μm	8 to 25μm	6 to 36μm	10,000 to 500,000 particles/mL

Description

• Engineered with a micro-fabricated, cell-sensing zone that enables discrimination by cell size and cell volume - down to sub-micron and sub-picoliter resolution
• Has a precision drilled aperture through which cells pass and are counted
• Counts based on Coulter principle of impedance changes across current established by sensor electrodes
• Ensures reproducible counts by drawing precisely 50μL of sample into its liquid handling microchannel each time
• Eliminates pipetting errors and uneven sample loading with precision liquid handling channels
• 40μm-aperture sensor
  • Able to count cells between 4 and 12μm in diameter and particles between 3 and 17μm
• 60μm-aperture sensor
  • Able to count cells between 8 and 25μm in diameter and particles between 6 and 36μm

Specifications

• Product Type: Scepter Sensors
• Memory: Stores up to 72 counts
• Test Methods: Automated
• For Use With (Equipment): Scepter 2.0 handheld automated cell counter

• Analysis Time: <30 sec.
• Sample Type: CHO cells, HeLa cells and mouse embryonic stem cells, can even be used to count subpopulations in peripheral Blood mononuclear cell preps
• For Use With (Application): Counting cells and delivering histrogram profiles of cellular populations