Endothelin 1 Monoclonal Antibody (TR.ET.48.5), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA3005

Product Code. 11555722

Description

MA3-005 detects ET-1 from human, sheep, mouse and rat tissues. This antibody shows little cross-reactivity to related peptides and no known cross-reactivity to non-related peptides. MA3-005 has been successfully used in Western blot, flow cytometry, immunofluorescence, immunohistochemistry, immunocytochemistry, and radioimmune assay procedures. Immunohistochemical staining of ET-1 in human corpus cavernosum tissue with MA3-005 results in staining of endothelial cells. Radioimmune assays using MA3-005 can be used to concentrate ET-1 in solution (e.g. serµm/plasma, milk, urine). This antibody is not recommended for human placenta tissue or HepG2 cells in Western blot applications. For immunohistochemistry with MA3-005 formalin-fixed, paraffin-embedded tissue sections has been used. The MA3-005 antigen is purified human endothelin-1. Studies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16.

The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly

Specifications

• Antigen: Endothelin 1
• Classification: Monoclonal
• Concentration: 2.5 mg/mL
• Formulation: PBS with 0.05% sodium azide
• Gene Accession No.: P05305, P22387, P22388, Q9BG76
• Gene Symbols: EDN1
• Immunogen: Pure endothelin-1 conjugated to KLH.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Ovine, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Radioimmune Assays (RIA), Western Blot
• Clone: TR.ET.48.5
• Conjugate: Unconjugated
• Gene: EDN1
• Gene Alias: ARCND3; Big endothelin 1; Big endothelin-1; Big ET; Big ET 1; Big ET1; BigET; EDN1; endothelin 1; endothelin-1; Et; ET1; ET-1; HDLCQ7; PPET1; ppET-1; preproendothelin; preproendothelin-1; preproET; QME
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 13614, 1906, 24323, 443498
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid