Gibco™ ExpiFectamine™ 293 Transfection Kit

Designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells.

Brand:  Gibco™ A14524

Product Code. 13385544

Description

The ExpiFectamine™ 293 Transfection Kit is a core component of the Expi293™ Expression System. It is designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells. The high-efficiency, cationic, lipid-based transfection reagent and transfection enhancers are designed to power the highest possible level of protein expression from Expi293F™ cells cultured in Expi293™ Expression Medium

• Designed specifically for transfection of high density suspension cell culture, with matching transfection enhancers that boost transfection performance and protein expression
• Achieves protein yields 2- to 10-fold higher than other transfection reagents used on high density 293 cell cultures
• Employs the same transient expression protocols typically used in current low-density 293 suspension culture systems to easily switch from low density systems to the high yield, high density Expi293™ Expression System
• Provides robust and reproducible transfection results, giving users greater confidence in their results
• Scale transfections for culture volumes of less than 1 mL to greater than 10 liters, while maintaining equivalent volumetric protein yields

The ExpiFectamine™ 293 Transfection Kit is specifically designed to work with Expi293F™ cells in the Expi293™ Expression System. The transfection kit consists of the cationic lipid-based ExpiFectamine™ 293 Reagent and ExpiFectamine™ 293 Transfection Enhancers 1 and 2. The enhancers are added 18 to 22 hours post-transfection to boost transfection, cell viability, and protein expression. The protocol for use of ExpiFectamine™ 293 Transfection components is very similar to transfection protocols for other transient expression reagents, such as 293fectin™ and FreeStyle™ MAX.

Specifications

• Cell Line: Expi293F™
• Content And Storage: 2.7 mL (3 x 0.9 mL) ExpiFectamine™ 293 Reagent
  5 mL ExpiFectamine™ 293 Transfection Enhancer 1
  50 mL ExpiFectamine™ 293 Transfection Enhancer 2
  
  Sufficient for transfection of 1 L of culture
  
  Store at 2°C to 8°C
• Sample Type: Plasmid DNA
• For Use With (Application): Transfection
• Product Type: Transfection Kit
• Quantity: For 1 L of culture

• Classification: Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
• Cell Type: Established Cell Lines
• Transfection Technique: Lipid-based Transfection
• Product Line: ExpiFectamine™
• Purity: Cell Culture Grade
• Serum Compatible: No

Safety and Handling

Product Identifier

• ExpiFectamine 293 Transfection Kit

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H302-Harmful if swallowed.
• H312-Harmful in contact with skin.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.
• H335-May cause respiratory irritation.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Supplemental information

• MIXTURE LIST-Contains : 3-Fluoro-4-nitrophenol

For Research Use Only. Not for use in diagnostic procedures.