SuperScript™ EXPRESS One-Step Superscript™ qRT-PCR Kit, with premixed ROX

Next-generation, real-time qRT-PCR reagents designed to complement fast-cycling instruments. Get the turnaround time you want with the data quality you need.

Product Code	Brand	No. of Reactions
10574414	SuperScript™ 11791200	500 Reactions
10174783	SuperScript™ 1179101K	2500 Reactions

Description

EXPRESS One-Step SuperScript™ qRT-PCR SuperMix Kits incorporate proven Invitrogen enzymes now specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for your probe-based qPCR experiments.

• Fast-activating, antibody-mediated Platinum™ Taq DNA Polymerase - complete, rapid activation provides the best sensitivity and reproducibility
• The One-Step SuperScript™ III Reverse Transcriptase module has a unique formulation to ensure better yield and less PCR inhibition, specifically for one-step qRT-PCR
• Flexible formats with ROX premixed or separate - designed to work with default instrument protocols on a wide range of high-throughput fast instruments (e.g., AB 7500 Fast, AB 7900 HT Fast, AB StepOne, Roche LightCycler™ 480, Corbett Rotor-Gene™)
• The only commercially available one-step qRT-PCR kits containing heat-labile UDG - significantly reduced carryover contamination risk with excellent activity from room temperature to approximately 40°C. Due to the thermostability of SuperScript™ III, the cDNA synthesis step is run at 50°C, at which temperature the heat-labile UDG is totally and irreversibly inactivated

Easy to test and switch:

• EXPRESS kits work with manufacturer's default protocols on fast instruments and come in a convenient and stable SuperMix format for ultimate convenience
• The kits are offered with either premixed ROX or a separate tube of ROX to fit your instrument needs

Order Info

Guaranteed to be stable for 6 months when properly stored.

Specifications

• Content And Storage: EXPRESS One-Step SuperScript™ qRT-PCR SuperMixes are composed of 2 modules: an EXPRESS RT module (containing SuperScript™ III Reverse Transcriptase and RNAseOUT™) and an EXPRESS qPCR SuperMix module with ROX premixed or in a separate tube.
  
  Store at -20°C upon receipt. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteed stable for 6 months when properly stored.
• Format: Tube
• Polymerase: Taq DNA Polymerase
• For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, StepOne™, Fast Mode, StepOne™, Standard Mode
• Product Type: qRT-PCR Kit
• Sample Type: RNA

• Detection Method: Primer-probe
• PCR Method: 1-step RT-qPCR
• Reaction Speed: Fast or Standard
• Product Line: SuperScript™
• For Use With (Application): Gene Expression

For Research Use Only. Not for use in diagnostic procedures.