Thermo Scientific™ EZ-Link™ Maleimide-PEG4-DBCO

A sulfhydryl reactive heterobifunctional crosslinker which contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications. The thiol/sulfhdryl reactive maleimide can be used to label a variety of thiol containing moieties such as free cystines in proteins, peptides, or other (bio)molecules.

Product Code	Brand	Quantity
17611586	Thermo Scientific™ C20041	25 mg
17631586	Thermo Scientific™ C20044	10 x 1 mg

Description

• A sulfhydryl reactive heterobifunctional crosslinker which contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications
• The thiol/sulfhdryl reactive maleimide can be used to label a variety of thiol containing moieties such as free cystines in proteins, peptides, or other (bio)molecules
• Once a protein or (bio)molecule is DBCO labeled, it can undergo a copper-free azide-alkyne cycloaddition reaction to form a stable triazole linkage with an azide labeled coupling partner
• Can be used to create diverse conjugates with the biorthogonal coupling between an alkyne and azide (supplied separately)
• The coupling or crosslinking procedure is typically done in two steps:
  • First, a protein or thiol containing molecule is labeled with a DBCO group and excess crosslinker is removed
  • Second, an azide containing moiety (prepared independently) is introduced into the same system as the DBCO moiety
  • Labeling occurs under biological conditions without the introduction of any additional reagents such as a copper catalyst
• Reagent readily dissolves in water-miscible solvents for dilution into aqueous reaction mixtures

Specifications

• Chemical Reactivity: Azide-Sulfhydryl
• Description: Maleimide-PEG4-DBCO
• PEGylated: Yes
• Content And Storage: Upon receipt store desiccated at -20°C.
• Shipping Condition: Wet Ice
• Labeling Method: Chemical Labeling
• Reactive Moiety: Maleimide, DBCO
• Solubility: DMF, DMSO
• Water Soluble: No

• Cleavable: No
• Molecular Weight (g/mol): 647.74
• Spacer Arm Length: 29.7 Å
• Cell Permeability: Not determined
• Product Line: EZ-Link™ No-Weigh™
• Crosslinker Type: Heterobifunctional
• Spacer: Medium (10 to 30 Å)
• Format: Standard, Single-use
• Product Type: Crosslinker