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Thermo Scientific™ EZ-Link™ Psoralen-PEG3-Biotin
Biotinylate DNA or RNA via intercalation and conjugation with thymine- and other pyrimidine-containing bases with this light-activated biotinylation reagent.
Brand: Thermo Scientific™ 29986
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Description
Thermo Scientific™ EZ-Link Psoralen-PEG3-Biotin enables biotinylation of DNA or RNA via UV-light-activated intercalation of the psoralen group with thymine- and other pyrimidine-containing bases to form covalent bonds.
Highlights:
- Water-soluble, polyethylene glycol (PEG) spacer arm
- Much higher coupling yield than achieved with photoactivatable biotin
- Psoralen moiety reacts through a cyclo-addition mechanism with the 5,6 double bond in thymine- and other pyrimidine-containing bases
- Covalent attachment occurs with exposure to UV light (approximately equal to 350nm)
- Great for biotinylating DNA/RNA probes (∽1 biotin/10-20 bases)
- Ultrahigh sensitivity relative to radiolabeled probes (<100 femtograms)
- Does not interfere with hybridization
Specifications
| Psoralen-PEG3-Biotin | |
| Store at 4°C protected from light and moisture. Shipped at ambient temperature. | |
| Cell-Impermeant | |
| Psoralen | |
| Biotin | |
| Biotin & Analogs |
| 5 mg | |
| EZ-Link™ | |
| Light-activated | |
| DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water | |
| Extra-long, Pegylated |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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