Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit

Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Brand:  Thermo Scientific™ 21435

Product Code. 10117773

Description

Thermo Scientific™ EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Highlights:

• Water-soluble analog of NHS-LC-Biotin
• Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate or phosphate buffers
• Negative charge of -SO₃ group keeps biotinylation localized at the cell surface
• Creates an amide bond

U.S. Patents 5,892,628 and 5,872,261.

Specifications

• Description: EZ-Link Sulfo-NHS-LC-Biotinylation Kit
• Quantity: 10 reactions
• Labeling Scale: 1 to 10 mg
• Solubility: Water
• Labeling Target: Antibodies, Proteins

• Product Type: Sulfo-NHS-LC-Biotinylation Kit
• Content And Storage: Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody
  • EZ-Link Sulfo-NHS-LC-Biotin, 25 mg
  • PBS Pack (makes 500 mL), 1 pack
  • Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns
  • HABA Solution, 1 mL
  • Avidin, 10 mg
  
  Store biotin and avidin reagents at -20°C. Store remaining kit components at 4°C.
• Product Line: EZ-Link™
• Label or Dye: Biotin

Safety and Handling

Product Identifier

• Sulfo-NHS-LC-Biotinylation Kit

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2

Hazard Statement

• H319-Causes serious eye irritation.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Supplemental information

• MIXTURE LIST-Contains : Sodium chloride, Sodium phosphate dibasic

For Research Use Only. Not for use in diagnostic procedures.