Thermo Scientific™ EZ-Link™ TFP Ester-PEG4-DBCO

An amine reactive heterobifunctional crosslinker which contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications. The crosslinker can be used to label a variety of amine containing molecules such as proteins, biomolecules, or small molecules.

Product Code	Brand	Quantity
17514346	Thermo Scientific™ C20039	10 x 1 mg
17621586	Thermo Scientific™ C20043	10 x 1 mg

Description

• An amine reactive heterobifunctional crosslinker which contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications
• The crosslinker can be used to label a variety of amine containing molecules such as proteins, biomolecules, or small molecules
• Once a protein or (bio)molecule is DBCO labeled, it can undergo a copper-free azide-alkyne cycloaddition reaction to form a stable triazole linkage with an azide labeled coupling partner
• Can be used to create diverse conjugates with the biorthogonal coupling between an alkyne and azide (supplied separately)
• The coupling or crosslinking procedure is typically done in two steps
  • First, a protein or amine containing molecule is labeled with a DBCO group and excess crosslinker is removed
  • Second, an azide containing moiety (prepared independently) is introduced into the same system as the DBCO moiety
  • Labeling occurs under biological conditions without the introduction of any additional reagents such as a copper catalyst
• Ragent readily dissolves in water-miscible solvents for dilution into aqueous reaction mixtures
• TFP esters are more hydrolytically stable than NHS esters

Specifications

• Chemical Reactivity: Amine-Azide
• Description: TFP Ester-PEG4-DBCO
• PEGylated: Yes
• Content And Storage: Upon receipt store desiccated at -20°C.
• Shipping Condition: Wet Ice
• Labeling Method: Chemical Labeling
• Reactive Moiety: TFP Ester, DBCO
• Quantity: 10 x 1 mg
• Format: Standard, Single-use
• Product Type: Crosslinker

• Cleavable: No
• Molecular Weight (g/mol): 714.7
• Spacer Arm Length: 17.9 Å
• Cell Permeability: Not determined
• Product Line: EZ-Link™ No-Weigh™
• Crosslinker Type: Heterobifunctional
• Spacer: Medium (10 to 30 Å)
• Solubility: DMF, DMSO
• Water Soluble: No