Invitrogen™ eBioscience™ eZKine™ CD8 Activation 1 Whole Blood Intracellular Cytokine Kit

Designed to rapidly identify cytokine producing T lymphocytes of the CD8+ T cell lineage after stimulation of whole peripheral blood samples.

Brand:  Invitrogen™ 8822-6854-41

Product Code. 15551297

Includes:

• eZKine CD8 Activation 1 Cocktail : 25 tests. This cocktail contains the following antibodies:
• Anti-Human CD3 (SK7) APC
• Anti-Human CD8 (SK1) PerCP-eFluor 710
• Anti-Human CD69 (FN50) PE
• Anti-Human IFN gamma (4S.B3) FITC
• eZKine Isotype Control Cocktail D : 25 tests. This cocktail contains the following antibodies:
• Anti-Human CD3 (SK7) APC
• Anti-Human CD8 (SK1) PerCP-eFluor 710
• Mouse IgG1 K Isotype Control PE
• Mouse IgG1 K Isotype Control FITC
• eZKine Fix/Lyse Concentrate (4X)
• eZKine Fix/Lyse Diluent
• Permeabilization Buffer (10X)

Description

• Stimulation of whole blood in the presence of Protein Transport Inhibitor Cocktail (cat. 00-4980, not included) is followed by red blood cell lysis and fixation in a single step and subsequent permeabilization.
• Samples are then ready to stain with the CD8 Activation 1 cocktail and concentration-matched Isotype Control cocktail.

Specifications

• Reactivity: Human
• Detects: CD3, CD69, CD8, IFN gamma
• For Use With (Application): Intracellular Staining Followed by Flow Cytometric Analysis
• Content And Storage: 2°C to 8°C. Do not freeze. Light sensitive material.