Thermo Scientific™ TBE Buffer (Tris-borate-EDTA) (10X)

Perform electrophoresis of nucleic acids in agarose and polyacrylamide gels using Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA).

Brand:  Thermo Scientific™ B52

Product Code. 10130740

Description

TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels, but is not recommended for preparative gels for recovery of nucleic acids. Since borate in TBE buffer is a strong inhibitor for many enzymes, TAE buffer (Tris Acetate-EDTA buffer, 10X powder, sc-296647) is recommended when looking at enzymatic applications for the DNA sample.

Usage Recommendations

• Use fresh 1X TBE both for the gel and for the electrophoresis run
• To prepare 1X TBE buffer, add 100mL of 10X TBE buffer to 900mL of deionized water and mix well

Quality Control

• The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests

1X Composition

• 89mM Tris, 89mM boric acid, 2mM EDTA, pH of 10X TBE: 8.3

Recommended for:

Electrophoresis of nucleic acids in agarose and polyacrylamide gels; Used both as a running buffer and as a gel preparation buffer; Filtered through a 0.22μm membrane; Recommended for electrophoresis of RNA and DNA fragme.

Note:

Double-stranded linear nucleic acid molecules migrate about 10% slower in TBE buffer than in TAE buffer.

Specifications

• Concentration: 10X
• Quantity: 1 L
• Product Type: TBE Buffer

Safety and Handling

Product Identifier

• TBE Buffer (Tris-borate-EDTA) (10X)

Signal Word

• Danger

Hazard Category

• Reproductive toxicity Category 1B

Hazard Statement

• H360-May damage fertility or the unborn child.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Boric acid

For Research Use Only. Not for use in diagnostic procedures.