Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)

Thermo Scientific™ DreamTaq DNA Polymerase is an enhanced TaqDNA polymerase optimized for all standard PCR applications.

Brand:  Thermo Scientific™ EP0703

Product Code. 11883813

Description

Perform all standard PCR applications with Thermo Scientific™ Fermentas DreamTaq™ DNA Polymerase, an enhanced Taq DNA polymerase.

DreamTaq DNA Polymerase ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. DreamTaq DNA Polymerase uses the same reaction set-up and cycling conditions as conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required. It is supplied with optimized DreamTaq buffer, which includes 20 mM MgCl₂. DreamTaq DNA Polymerase generates PCR products with 3'-dA overhangs. It does not incorporate dUTP.

• Robust amplification with minimal optimization
• High yields of PCR products
• Higher sensitivity compared to conventional Taq DNA polymerase
• Amplification of long targets up to 6kb from genomic DNA and up to 20kb from viral DNA
• Generates 3'-dA overhangs
• Incorporates modified nucleotides

Definition of Activity Unit

• One unit of the enzyme catalyzes the incorporation of 10nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 min at 70°C
• Enzyme activity is assayed in the following mixture: 67mM Tris-HCl (pH 8.8 at 25°C), 6.7 mM MgCl₂, 1mM 2-mercaptoethanol, 50mM NaCl, 0.1mg/mL BSA, 0.75mM activated calf thymus DNA, 0.2mM of each dNTP, 0.4MBq/mL [3H]-dTTP

Storage Buffer

• The enzyme is supplied in: 20mM Tris-HCl (pH 8.0), 1mM DTT, 0.1mM EDTA, 100mM KCl, stabilizing agent and 50% (v/v) glycerol

Quality Control

• The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests; Functionally tested in PCR

Inhibition and Inactivation

• Inhibitors: ionic detergents (deoxycholate, sarkosyl and SDS) at concentrations higher than 0.06, 0.02 and 0.01%, respectively (1)
• Inactivated by phenol/chloroform extraction

Recommended for:

Routine PCR amplification of DNA fragments up to 6kb; RT-PCR; Genotyping; Generation of PCR products for TA cloning

Specifications

• Content And Storage: Contains:
  • 5 x 100 μL DreamTaq DNA Polymerase (5 U/μL)
  • 10 x 1.25 mL 10X DreamTaq Buffer (includes 20 mM MgCl₂)
  
  Store at -20°C.
• Polymerase: DreamTaq DNA Polymerase
• Quantity: 5 x 500U (5U/μL)
• Hot Start: No
• Reaction Format: Standalone

• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Fidelity (vs. Taq): 1 X
• Overhang: 3'-A

For Research Use Only. Not for use in diagnostic procedures.