Thermo Scientific™ GeneJET Gel Extraction and DNA Cleanup Micro Kit

The GeneJET Gel Extraction and DNA Cleanup Micro Kit effectively removes primers, primer dimers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures.

Brand:  Thermo Scientific™ K0831

Product Code. 13253149

Description

The kit combines the convenience of spin column technology with the selective binding properties of a silica membrane, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

The GeneJET Gel Extraction and DNA Cleanup Micro Kit effectively removes primers, primer dimers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures. The kit can be used for purification of DNA fragments from 100 bp to 20 kb. The recovery rates are 90 to 100% for 100 bp to 3 kb DNA fragment size range. Each GeneJET DNA Purification Column has a total binding capacity of up to 10 μg of DNA, and the entire procedure takes approximately 3.5 minutes for DNA cleanup

Thermo Scientific GeneJET Gel Extraction and DNA Cleanup Micro Kit is developed as 3 in 1 kit designed for rapid and efficient purification of DNA from PCR, enzymatic reaction mixtures, and DNA extraction from standard or low-melting point agarose gels run in either Tris acetate (TAE) or Tris borate (TBE) buffer. The kit combines the convenience of spin column technology with the selective binding properties of a silica membrane, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

The GeneJET Gel Extraction and DNA Cleanup Micro Kit effectively removes primers, primer dimers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures. The kit can be used for purification of DNA fragments from 100 bp to 20 kb. The recovery rates are 90 to 100% for 100 bp to 3 kb DNA fragment size range. Each GeneJET DNA Purification Column has a total binding capacity of up to 10 μg of DNA, and the entire procedure takes approximately 3.5 minutes for DNA cleanup

Highlights
• Advanced column design—easy to open, load and handle
• Yields concentrated DNA - Elute in 6 to 10 μL volume
• High recovery - 90 to 100% recovery in the 100 to 3000 bp DNA fragment range
• Fast—4 minute reaction mixture clean-up and 15 minute agarose gel purification protocols available
• Efficient removal of reaction contaminants - including enzymes, proteins, primers dimers, dNTPs, and salts
• Flexible—does not require precise gel slice quantification weighting

Applications
The purified DNA can be used in common downstream applications:
• Sequencing
• Restriction digestion
• PCR
• qPCR
• Labeling
• Ligation
• Cloning
• In vitro transcription
• Blotting
• In situ hybridization

Includes
• Binding Buffer
• Extraction Buffer
• Prewash Buffer (conc.)
• Wash Buffer (conc.)
• Elution Buffer
• DNA Purification Micro Column & Collection Tube

Specifications

• Column Type: Spin Column
• Isolation Technology: Silica Column
• For Use With (Application): Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Nucleic Acid Labeling, Sequencing, PCR, In Vitro Transcription
• Product Line: GeneJET
• Quantity: 50 Preps
• Test Time: 15 min.

• Format: Spin Column
• Sample Type: DNA, Gel Samples
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Product Type: Gel Extraction and DNA Cleanup Kit
• Target: DNA from Enzymatic Reactions (e.g. PCR)

Safety and Handling

Product Identifier

• GeneJET Gel Extraction and DNA Cleanup Micro Kit

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Skin corrosion/irritation Category 2
• Serious eye damage/eye irritation Category 2
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H412-Harmful to aquatic life with long lasting effects.
• EUH032-Contact with acids liberates very toxic gas.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P321-Specific treatment (see on this label).
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P330-Rinse mouth.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P501-Dispose of contents/container to .

Supplemental information

• MIXTURE LIST-Contains : guanidinium thiocyanate, guanidinium chloride

For Research Use Only. Not for use in diagnostic procedures.