Thermo Scientific™ GeneJET Gel Extraction Kit

Thermo Scientific GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.

Brand:  Thermo Scientific™ K0692

Product Code. 10670091

Description

Thermo Scientific GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.

The kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column. The kit can be used to purify DNA fragments from 25 bp to 20 kb in size with recovery rates up to 95%. Each GeneJET purification column has a binding capacity of up to 25 μg of DNA and can process up to 1 g of agarose gel. The entire procedure takes just 15 minutes, and the isolated DNA is ready to use in all common downstream applications, including ligation, restriction digestion, PCR, sequencing, and labeling.

Highlights
• Highly efficient: up to 95% recoveries in the range of 100 bp to 10 kb
• Fast: procedure takes only 15 minutes
• Convenient: spin columns are capped and assembled with collection tubes

Applications
Fast and efficient extraction of high purity DNA fragments ideal for use in all conventional molecular biology procedures including:
• FastDigest or conventional restriction digestion
• Automated fluorescent or radioactive sequencing
• PCR
• In situ hybridization
• Labeling
• Cloning

Specifications

• Column Type: Spin Column
• Isolation Technology: Silica Spin Column
• Elution Volume: 50 μL
• For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
• Quantity: 250 Preps
• Test Time: 15 min.
• Starting Material Amount: ≤1 g

• Content And Storage: • 30 mL Binding Buffer; room temperature
  • 9 mL Wash Buffer (concentrated); room temperature
  • 15 mL Elution Buffer; room temperature
  • 50 GeneJET Purification Columns with Collection Tubes; room temperature
  
  
• Sample Type: Agarose gels
• Final Product Type: DNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Shipping Condition: Room Temperature
• Yield: 25 μg (Binding capacity)

Safety and Handling

Product Identifier

• genejet gel extr 250 prep

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Skin corrosion/irritation Category 1 C

Hazard Statement

• H302-Harmful if swallowed.
• H314-Causes severe skin burns and eye damage.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P330-Rinse mouth.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : thiocyanic acid

For Research Use Only. Not for use in diagnostic procedures.