Thermo Scientific™ GeneJET Plasmid Maxiprep Kit

Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column.

Brand:  Thermo Scientific™ K0492

Product Code. 11561645

Description

GeneJET Plasmid Maxiprep Kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 μg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 μg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights
• Efficient—high yields up to 750 μg of high quality plasmid DNA from 250 mL of bacterial culture
• High concentration of DNA purified— >400 ng/μL
• Convenient—spin column format, centrifuge or vacuum protocols
• Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications
Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
• Transfection of robust cell lines
• FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
• In vitro transcription

Specifications

• Concentration: 400 ng/μL
• Format: Spin Column
• Sample Type: Bacteria
• For Use With (Application): Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR
• No. of Reactions: 25 Preps
• Product Line: GeneJET
• Target: Plasmid DNA

• Column Type: Spin Column
• Isolation Technology: Silica Column
• Scale: Maxi
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Prep Scale: > 200 μg (Large-Scale) Plasmid DNA
• Product Type: Plasmid MaxiPrep Kit
• Test Time: 60 min.

Safety and Handling

Product Identifier

• GeneJET Plasmid Maxiprep Kit

Signal Word

• Danger

Hazard Category

• Skin corrosion/irritation Category 1 A
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H319-Causes serious eye irritation.
• H314-Causes severe skin burns and eye damage.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P373-DO NOT fight fire when fire reaches explosives.

Supplemental information

• MIXTURE LIST-Contains : Sodium lauryl sulfate, Sodium hydroxide,acetic acid, Sodiumlauryl sulfate, Sodium hydroxide

For Research Use Only. Not for use in diagnostic procedures.