Thermo Scientific™ GeneJET Plasmid Midiprep Kit

Designed for large-scale isolation of high quality plasmid DNA from recombinant E.coli cultures.

Brand:  Thermo Scientific™ K0482

Product Code. 11541645

Description

GeneJET Plasmid Midiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 μg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Thermo Scientific GeneJET Plasmid Midiprep Kit, sufficient for 25 preps, is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 μg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights
• Efficient—high yields up to 200 μg of high quality plasmid DNA from 50 mL of bacterial culture
• High concentration of DNA purified— >400 ng/μL
• Convenient—spin column format, centrifuge or vacuum protocols
• Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications
Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
• FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
• In vitro transcription
• Transfection of robust cell lines

Specifications

• Concentration: 400 ng/μL
• Format: Spin Column
• Scale: Midi
• For Use With (Application): Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR
• Prep Scale: 100-200 μg (Medium-Scale) Plasmid DNA
• Product Type: Plasmid MidiPrep Kit
• Target: Plasmid DNA

• Column Type: Spin Column
• Isolation Technology: Silica Column
• Sample Type: Bacteria
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Product Line: GeneJET
• Quantity: 100 Preps
• Test Time: 60 min.

Safety and Handling

Product Identifier

• GeneJET Plasmid Midiprep Kit

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : acetic acid, tritonX-100, guanidine hydrochloride

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For Research Use Only. Not for use in diagnostic procedures.