Thermo Scientific™ Kpn2I (BspEI) (10 U/μL)

The Kpn2I (BspEI) restriction enzyme recognizes T^CCGGA sites and cuts best at 55°C in Tango buffer (Isoschizomers: AccIII, Aor13HI, BseAI, Bsp13I, BspEI, MroI).

Product Code	Brand	Quantity
10150830	Thermo Scientific™ ER0531	500 U
10703721	Thermo Scientific™ ER0532	2500 U

Description

Lambda DNA digested with Kpn2I (BspEI), 0.7% agarose, 24 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

• Superior quality -stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Specifications

• Concentration: 10 U/μL
• Enzyme: Kpn2I (BspEI)
• Sensitive to Heat Inactivation: Yes
• Type IIS RE: No
• Research Category: Traditional Cloning

• Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
• Compatible Buffer: 10X Buffer Tango
• Optimal Reaction Temperature: 55°C
• Product Type: Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures.